The African catfish is a commercially farmed fish with great potential to contribute to reducing the fish gap in Uganda. However, high mortalities in catfish hatcheries are a major concern as less than 150,000 catfish fry are produced out of estimated 30m catfish fingerlings that are required annually. Therefore, this first Edition of the Manual is intended for use by Uganda's operators of the African catfish hatcheries. It provides basic facts about catfish developmental stages, the recommended live feed (i.e. Moina, a small microscopic water organism). The Manual also provides the hatchery operators with procedures and a step wise process to produce and sustain large quantities of Moina for a commercial scale enterprise.