Azabo R, Abila P, Odhiambo L, Waiswa C, Ejobi F, Okedi L., Trypanosome Infection rates in tsetse (Glossina fuscipes fuscipes) in communal grazing lands in Mid-northern Uganda, Onl J Vet Res., 20(4):225-236, 2016. Glossina fuscipes fuscipes is the main tsetse species of economic importance in Uganda as it plays a major role in transmission of trypanosomosis. Epidemiology in livestock is determined by prevalence and distribution of arthropod vectors in affected areas and proportion of infected tsetse flies. We found tsetse apparent densities varied between 0.21-2.13 flies/trap/day across 3 study areas with Glossina fuscipes fuscipes being the only species. 376 non teneral flies were trapped, dissected with proboscis, salivary gland and mid-gut for microscopical determination of trypanosome infection. 21 (5.6%) flies were infected with trypanosomes, significantly (x2 , 8.05, d.f.= 1,P=0.002), higher in females (81%) than males (19%). Higher infection rates occurred during wet (3.7%), than dry season (1.9%). Infections were due to T. vivax (3.2%), T. congolense (1.3%), and 1.1% for immature (gut only). T. brucei was not isolated. Blood meal analysis by PCR implicated cattle and human hosts.