Item Details

Title: Virulence diversity of colletotrichum lindemuthianum in uganda

Date Published: 2014
Author/s: Kiryowa M, A. Ebinu A, Kyaligonza V, Nkalubo S, Paparu P, C. Mukankusi C, and P. Tukamuhabwa P
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Journal Publisher: Journal of Agricultural Research
Keywords: Bean anthracnose, resistance genes, races.


Colletotrichum lindemuthianum is a highly variable pathogen of common beans that easily defeats resistance in cultivars bred with single-gene ressistance. To determine virulence variability of the pathogen in Uganda, samples of common bean tissues with anthracnose symptoms were collected in eight districts of Uganda, namely Kabarole, Sironko, Mbale, Oyam, Lira, Kapchorwa, Maracha and Kisoro. 51 isolates sporulated successfully on potato dextrose agar and Mathur’s media and were used to inoculate 12 differential cultivars under controlled conditions. Five plants per isolate for each cultivar were inoculated and then evaluated for their reaction using the 1 – 5 severity scale of Drifjhout and Davis (1989). Data was subjected to analysis of variance and races were classified using the binary nomenclature system proposed by Pastor Corrales (1991). Variation due to cultivar and isolate effects was highly significant (P = 0.001) for severity. The 51 isolates from eight districts grouped into 27 different races. Sironko district had the highest number of races followed by Mbale and Kabarole. Races 2047 and 4095 were the most frequently found, each with 10 isolates grouped under them. Race 4095 had the broadest spectrum of virulence since it caused a susceptible (S) reaction on all 12 differential cultivars and the susceptible check. This was followed by races 2479, 2047 and 2045 respectively. Two races, 4094 and 2479, caused a susceptible reaction on the highly resistant differential cultivar G2333, which nevertheless, showed the broadest resistance followed by Cornell, TU, and AB136 respectively. These cultivars are recommended for use in breeding programs aimed at breeding for broad resistance to bean anthracnose in Uganda.