Understanding the nature of variability of traits is important to select the breeding scheme to be employed for the improvement of the target trait. Three local rosette resistant lines belonging to Spanish [ICGV-SM 99566 and ICGV-SM 96801] and Virginia [ICGV-SM 90704] groups were crossed with two exotic susceptible lines belonging to Valencia group [M3 and Valencia C]. A total of six populations were generated from each cross, these included P1 [resistant], P2 [susceptible], F1, F2 and BC1P1, BC1P2. They were evaluated in the field using the infector row technique where the source of the innoculum was a susceptible local variety Acholi white. The results showed significant varying levels of resistance to GRD among the early segregating generations [F2 and backcrosses] of improved groundnut populations. All the six F1's were found resistant to GRD [mean score range [1.67 to 2.00] indicating that resistance is governed by dominant genes and the mean disease score in F2 were generally more resistant than the backcrosses to the elite [M3 and Valencia C] parents but the resistance of F2 was comparable to the backcrosses of the donor [ICGV-SM 90704, ICGV-SM 99566 and ICGV-SM 96801] parents. The donor parents [ICGV-SM 90704, ICGV-SM 99566 and ICGV-SM 96801] had a range of 1-3 GRD score while the recipients parents [M3 and Valencia C] had a range of 7-9 GRD score indicating that both ICGV-SM 99566 and ICGV-SM 96801 should be considered as key resistant lines in addition to ICGV-SM 90704 that is commonly used in Uganda and other countries. Therefore, this study aimed at establishing the different levels of resistance to groundnut rosette disease [GRD] in the segregating populations so as to determine the selection procedure for advancement of the populations that are resistant to the groundnut rosette disease.